How To Microdose 4-AcO-DMT? How to Take 4-AcO-DMT Microdose?

Everything You Need To Know About Microdosing 4-AcO-DMT (Psilacetin). To prepare 4-AcO-DMT for microdosing, start by measuring out 10mg on an electronic milligram scale. Add this to 100ml of ethanol (Everclear). Now 25ml of this solution will contain 2.5mg of 4-AcO-DMT, as 100ml divided by 4 is 25ml, and 10mg divided by 4 is 2.5mg

How To Microdose 4-AcO-DMT? How to Take 4-AcO-DMT Microdose?

Table Of Contents

Here is Psychedelics Daily’s guide on how to Microdose 4-AcO-DMT. If you are unfamiliar with 4-AcO-DMT, please read our guide on What is 4-AcO-DMT?, otherwise known as Psilacetin or O-Acetylpsilocin first. In that articl we also discuss the legality of 4-Aco-DMT in the world.

Microdosing 4-AcO-DMT

This is everything you’ve ever wanted to know about how to microdose 4-AcO-DMT, such as How to Take 4-AcO-DMT Microdose? What is 4-AcO-DMT? How to take a safe microdose? What microdosing schedule to use for 4-AcO-DMT?

We will show you how to prepare 4-AcO-DMT for microdosing and storage for long term using Ethanol (Everclear or Vodka) and how to calculate the proper dosage. We will also show you the correct dosage chart as compared to Psilcybin mushrooms.

What is 4-AcO-DMT?

In short, 4-AcO-DMT is a Psilocybin analogue by Dr. Albert Hofmann. He synthesized the molecule for stability by replacing the Hydorxyl/Phosphoryloxy group with an Acetyl group. It’s a popular alternative to psilocybin mushrooms. It is also known as Psilacetin and O-Acetylpsilocin.

If you are microdosing the 4-AcO-DMT substance, please contact us and comment below with your 4-AcO-DMT microdosing report.

What is a Microdose of 4-AcO-DMT?

Remember, from our Psychedelic Microdosing Guides, I mentioned in How to Microdose LSD and Magic Mushrooms?that a microdose is 10th to a 5th of the effective dose.

The effective dose of psilocybin is 2.5g. A tenth of 2.5g is a 0.25g (quarter gram). 4-AcO-DMT is the equivalent to Psilocybin mushrooms in the following ratio:

As discussed in the What is 4-AcO-DMT? guide, the effective normal dose of 4-AcO-DMT is about 25-mg. 10th of 25mg is 2.5mg.

1-dried-gram Psilocybin mushrooms is approx. equal to 10-mg of 4-AcO-DMT

What dose of 4-aco-DMT is best comparable to Psilocybin Microdosing?

2.5-mg of 4-AcO-DMT is equal to 0.25 gram Psilocybin Mushrooms.

The table below shows the amount in mg of 4 aco dmt is equivalent to a gram of shrooms:

According to Erowid, magic shrooms are roughly 0.5% psilocin by weight, so about 5mg per gram of dried shrooms. Psilocybin mushrooms contain 0.63% psilocybin and 0.60% psilocin. Therefore, 1-gram of shrooms has 6 mg each of psilocin and psilocybin. The psilocybin dephosphorylates to roughly 4.5mg of psilocin, which totals to approx 10mg of psilocin per gram of dried mushrooms.

The molar mass of 4-HO-DMT, or Psilocin is around 204.273 g/mol. Psilocybin’s molar mass is 284.25 g/mol.

4-AcO-DMT’s molar mass is about 246.3049 g/mol for freebase, and a molar mass of 116.07 g/mol for fumaric acid which adds to a total of 362 g/mol. 4-AcO-DMT is sold as fumarate salt or hydrochloride (HCL). The molar masses are 4-AcO-DMT HCl - 282.7649 g/mol, and 4-AcO-DMT Fumarate - 304.3399 g/mol respectively.

(362.4 / 204.3) * 5 = 8.869. We’ll round it off to 10. One gram of dried psilocybin mushrooms is roughly equal to about 10mg 4-AcO-DMT, for simplicity’s sake.

Psilocybin Mushrooms 4-AcO-DMT
Microdose - 0.25 (14) dried-grams 2.5-mg (Microdose)
0.5 (12) dried-grams 5-mg
1 dried gram 10-mg
2.5 dried-grams 25-mg
5 dried-grams 50-mg

4-AcO-DMT microdose is 2.5-mg. Start with 2.5-mg. Please keep in mind that these dosage levels were calculated by myself, which work best for my body. Some people have reported that 25-mg of 4-AcO-DMT feels like an eigth oz. (3.5 gram) mushroom trip.

Bearing in mind our unique physiologies, you may want to start your experiments with 1-mg. If you don’t notice any effects, try 1.5-mg. Similarly, if noticing no effects still, proceed to slowly increase the dosage to 2.5-mg.

If 2.5-mg produces no effects, then just as Psilocybin microdose is 0.25-grams to 0.5-grams, you may have to play around in the 2.5-mg to 5-mg range of 4-AcO-DMT as it is almost identical to Psilocybin Mushrooms.

Many people find that 4-AcO-DMT offers a much more consistent micro dose regimen because of the fact that unlike mushrooms, the potency of the compound isn’t affected by the amount. As mentioned in the Mushroom Dosage Calculator, Mushrooms vary in potency and in the psilocybin content due to strains and growing conditions. However, 2.5mg of 4-AcO-DMT is exactly 2.5mg of 4-AcO-DMT no matter what.

Microdosing 4-AcO-DMT offers more consistency than Psilocybin Mushrooms, many have found, including myself.

Preparing 4 AcO DMT Microdose

Without a scale that is able to measure in milligrams, 4-AcO-DMT can be very tricky to microdose. It is recommended that you get a scale, otherwise please do not attempt to consume 4-AcO-DMT, as eyeballing or miscalculating can turn out to be potentially dangerous. For harm reduction purposes, it is essential that you get a scale that can measure to the milligram, that is, to 0.001g.

If you don't have a milligram scale, do not attempt to consume 4-AcO-DMT fumarate or any powder form.

Buy a Milligram Scale

Accurate dosages can only be measures using an electronic milligram scale. If your measurement is off by even a few milligrams, the microdosing effects would be negated. You would either not take enough, or take too much.

Buy An Electronic Scale.

An electronic scale capable of measuring in milligrams can be found on Amazon for $15 to $25.

Microdosing 4-AcO-DMT Preperation

4-AcO-DMT Capsules

A lot of people don’t like the bitter taste, so they order empty capsules from Amazon and fill them up with the measured dose. It can be a bit tedious to individually measure out 2.5-mg, but some people don’t seem to mind the effort.

If you don’t have an accurate milligram scale, I don’t recommend consuming 4-AcO-DMT. Period.

Mixing 4 AcO DMT in Ethanol (Everclear)

To prepare 4-AcO-DMT for microdosing, start by measuring out 10mg on an electronic milligram scale. Add this to 100ml of Everclear Ethanol. Now, 25ml of this solution will contain 2.5mg of 4-AcO-DMT.

Please keep in mind that Ethanol is the best for mixing 4-AcO-DMT. Psilacetin oxydizes in water rather quicky for it to be a long term storage solution. Everclear or Vodka are the best option for psychedelic tryptamines, especially 4-substituted tryptamines, as they will oxidize rather quickly in distilled water.

Alcohol also ensures the solution remains free of any mold or bacterial growth. Many people like to use Everclear, Vodka, but almost any non-flavored alcohol solution which is greater than >20% would be fine for sterility and long term solution.

Psychonaut Wiki’s Volumetric Dosing Guide is one of the best methods by which to consume microdoses of 4-AcO-DMT, among many other substances.

If you don’t have a milligram scale, volumetric dosing will not work, you will not be able to measure a proper dose.

Mixing 4 AcO DMT in Drinks and Food

After having measured out the correct dosage, feel free to consume it with food or drinks per your taste by mixing in 25ml of the solution you prepared previously using 10mg of 4-AcO-DMT and 100ml distilled water. Apart from 4-AcO-DMT’s dose difference, almost everything mentioned in How to Prepare a Psilocybin Mushroom Microdose and How to Microdose Acid and Magic Mushrooms? is applicable. Follow those guides and stay safe.

Microdosing 4 AcO DMT Blotter Tabs

If you have measured blotter tabs of 4-AcO-DMT, start with finding out exactly what-mg they are. Usually, blotter tabs come in 20-mg - 25-mg. If that’s the case, and you have, for example, 20-mg blotter tabs of 4-AcO-DMT, then start by cutting the 20-mg tab into two equal parts to get 10-mg halves. Now divide these in half again to get four 5-mg squares. Now cut each 5-mg square in half to get 2.5-mg pieces. A microdose of 4-AcO-DMT is 2.5-mg.

What Is The Recommended Microdosing Schedule

Dr. Fadiman says to take a microdose every 4th day. For 10 weeks:

Dr. James Fadiman’s Microdosing Schedule

  • On day one, you dose.

  • Day two, you’re still having the effects.

  • Day three, you should be noticeably not having the effects, and on day four you dose again.

Dr. James Fadiman, author of The Psychedelic Explorer’s Guide and renowned psychedelic researcher
How to Microdose 4-AcO-DMT: Recommended Microdosing Schedule

How to Microdose 4-AcO-DMT: Recommended Microdosing Schedule

Please consult our How to Microdose LSD and Magic Mushrooms? guide, and the best microdosing schedule by Dr. James Fadiman to read more about the four-day microdosing schedule.

Dr Fadiman hasn’t spoken about 4-AcO-DMT microdosing to my knowledge. I have personally applied his regimen for mushrooms to 4-AcO-DMT and it seems to work exactly the same. In fact, after having microdosed on over a dozen 4-substituted tryptamines, I have found that almost all psychedelic tryptamines (with the notable exception of DMT) function in “almost” exactly the same manner in tiny doses such as microdoses.

Essentially, microdosing LSD or Psilocybin or 4-AcO-DMT (and many other psychedelic tryptamines drugs) in micro doses function and behave in exactly the same manner. I’ve observed for myself and I’ve not been able to verify these findings with others. Please send in your 4-AcO-DMT microdosing reports to us for further study. Thank you!

Be Mindful and Conscious While Microdosing

I find it essential to keep a daily journal of my activities, feelings, emotions, and thoughts. Be mindful and aware] as you observe your daily life. Read about how to prepare a psilocybin mushroom microdose.

Observe your thoughts. Be attentive to your mind and body. Observe any changes in your desire for various foods or pleasures. What do you find yourself wanting to do? I’ve observed that microdosing psychedelic tryptamines give me a clarity of perception with which I can observe the complete interaction of this living and thriving organism in the Universe in which it dwells.

In Conclusion, 4-AcO-DMT is Great For Microdosing

4-AcO-DMT is safe to microdose and consume. 2-mg to 2.5-mg (up to 5-mg) is the dosage range for microdosing 4-AcO-DMT. I find micorodosing 4-AcO-DMT to be more effecient and consistent than mushrooms. Due to the differences in mushrooms strains and growing conditions, their potency can vary. However, 10-mg of 4-AcO-DMT molecules are always exactly 10-mg of 4-AcO-DMT molecules.


Most of the research on 4-AcO-DMT was conducted on myself. When I started consuming 4-AcO-DMT circa 2008, I didn’t really know much about it. Luckily, I was able to find this forum post by Beeker which rather accurately described the dosage levels. Over the years, I have found these measurements to be on point and extremely accurate.

Please send in your 4-AcO-DMT microdosing reports to us for further study


Hash C. Borgir

Hash is a software developer, tech geek, and psychedelic explorer with a passion for writing about plant medicines, psychology, consciousness, Linux, web development, hacking and science related topics.