What Is 4-AcO-DMT?

4-AcO-DMT Essential Guide. Find out if 4-AcO-DMT is safe? How is 4-AcO-DMT used? What is a safe dosage of 4-AcO-DMT? What are the physical and psychological effects of 4-AcO-DMT? Is 4-AcO-DMT safe to use with other drugs?

What Is 4-AcO-DMT?

Table Of Contents

4-AcO-DMT Guide: Everything You Need To Know

What is 4-AcO-DMT? What is a safe dosage of 4-AcO-DMT? What are the physical and psychological effects of 4-AcO-DMT? Is 4-AcO-DMT safe to use with other drugs?

Can you microdose 4-AcO-DMT? Can you use it as a substitute for mushrooms? What is its tolerance? How does it compare to DMT? How does it compare to Psilocybin Mushrooms?

Psychedelics Daily’s guide to 4-AcO-DMT explores these questions. In addition, you can microdose 4-AcO-DMT!

I’ve personally microdosed this substance for 2 years. In the spirit of keeping this article more well-managed, I’ve written a much more detailed guide on How To Microdose 4-AcO-DMT.

What is 4-AcO-DMT?

4-AcO-DMT, otherwise known as 4-acetoxy-DMT, Psilacetin, and O-Acetylpsilocin is a synthetic 4 substituted psychedelic tryptamine. It is similar in structure to Psilocin and is actually a pro-drug for it.

Is Psilacetin safe? How is O-Acetylpsilocin used?

4-AcO-DMT is a popular psilocybi alternative

4-AcO-DMT is a popular psilocybi alternative

Acetylation of Psilocin by Hofmann

Acetylation is a chemical reaction which introduces an acetyl functional group into a chemical compound. This process results in what is known as an “Acetoxy” group.

This is a substitution of an acetyl group for an active hydrogen atom. Acetoxy group, shortened to AcO or O-Ac deacetylates upon first pass metabolism in the body.

Many drugs are acetylated to study their deacetylation effect on the body. In fact, a multitude of drugs and various other compounds require acetylation for effective metabolism.

4-AcO-DMT is the acetylated form of 4-HO-DMT (Psilocin) which itself is a metabolite of Psilocybin Mushrooms.

Psilocin was extracted, isolated, and acetylated by Dr. Albert Hofmann in the 60s. This is why 4-AcO-DMT is called 4 Acetoxy DMT as well.

History of 4-AcO-DMT

In 1963, Albert Hofmann employed by Sandoz Ltd. acetylated the psilocin compound. Even though the substance dates back to the 1960s, it has a limited history of human consumption. It wasn’t until a decade or so ago that 4-AcO-DMT came to light as a Research Chemical and as a pro-drug for Psilocin.

Dr. Hofmann acetylated the psilocin molecule, presumably in order for longer-term storage of psilocin. Psilocin may lose potency over time, however, Acetylated Psilocin in fumarate or HCL form don’t lose their efficacy.

Another reason for acetylation of the molecule was due to how acetylated drugs deacetylate and affect the metabolism of the drug.

What is the Legality of 4-AcO-DMT

Is 4-AcO-DMT legal? According to many sources online, such as the Psychonaut Wiki 4-AcO-DMT, the Legal Status of 4-AcO-DMT is as follows:

4-AcO-DMT is Illegal in:

  • Belgium

  • Brazil

  • Italy

  • Sweden

  • United Kingdom

4-AcO-DMT Is Either Not Illegal Or Unscheduled In The Following Countries

  • Germany (Illegal under Analog Act as it is an Ester of Psilocin)

  • United States: 4-AcO-DMT is unscheduled in the United States, however, 4-AcO-DMT is considered a Research Chemical or a designer drug sold by many online vendors. 4-AcO-DMT is an analog of psilocin and as such legal for labratory research. Possession of 4-AcO-DMT for the purposes of the sale for human consumption can be illegal under the Federal Analogue Act, under which any substance “substantially similar” to an illegal substance listed in Schedule I or II is treated like those in schedules, but only if intended for human consumption.

Pharmacology of 4-AcO-DMT

During the first pass metabolism and additional passes through the liver, 4-AcO-DMT is broken down or deacetylated into Psilocin. The Acetoxy group is broken off and only a hydroxyl remains on the fourth position. This rapid deacetylation to Psilocin is why 4-AcO-DMT hits faster and harder than mushrooms.

How Long Does 4-AcO-DMT Take

Many people have reported little difference between 4-AcO-DMT and Psilocybin Mushrooms other than the rapid onset. Where mushrooms are a gradual build up over an hour, O-Acetylpsilocin takes roughly 15-20 minutes.

The trip length is somewhat proportional to how much you ingest.

Effective Dose Affective Dosage Amount

4-AcO-DMT Dosage Amount
Threshold 5mg - 10mg
Light 10 - 15 mg
Common 25 - 30 mg
Strong 30 - 40+ mg
Heroic 50 + mg

How Long Does 4-AcO-DMT Last

4-AcO-DMT Duration Amount
Total Duration 6 - 8 hours
Come up + 20-60 minutes
Peak Effects + 1.5 - 2 hours
Come down + 3-5 hours
After-effects 2 - 4 hours

The TiHKaL entry on 4-AcO-DMT details various dosage levels in detail by Alexander and Ann Shulgin.

4-AcO-DMT’s Toxicity and LD-50

The Toxicity and LD-50 data on 4-AcO-DMT is lacking due to it’s lack of popularity and a limited history of human use. However, since it is a prodrug for Psilocin, it is very similar to the LD-50 of mushrooms. So far no deaths have been reported from this substance.

Those who have tried 4-AcO-DMT have yet to report any negative health issues associated with its use. I have personally eaten, drank, smoked, and vaporized 4-AcO-DMT in large amounts, even mixing it with large doses of Harmala, DMT, LSD, 25i-NBOME, Ketamine, Amanita Muscaris, Salvia Divinorum and many others, and I can report that it’s rather harmless when done within reason and balance.

Always start with low doses. You can always take more, later on. Harm reduction is about being conscious, aware and personally responsible.

4-AcO-DMT’s Dangerous Drug Interactions

(Source for Dangerous Interactions: Erowid, Tripsit, Psychonaut Wiki) (I made sure to simply avoid these and never bother. I don’t enjoy any of these drugs or the effects and feelings that they produce. I’ve never found any of these drugs to add anything to the experience or to my consciousness. I only like psychedelics.)

  • Alcohol

  • Antihypertensives (blood pressure medications)

  • Antipsychotics

  • Any CNS (Central Nervous System) depressants (Xanax, Ativan, etc)

  • Any drug containing dextromethorphan/DXM.

  • Lithium

  • Tramadol

  • Barbiturates

  • SSRIs or SSNRIs (avoid DMT if on anti-depressent medication)

  • Vasodilators

4-AcO-DMT Safe Drug Interactions

(Source for Safe Interactions: Personal Use and Research)

  • 25i-NBOME (As long as no MAOi was consumed)

  • 2CB (As long as no MAOi was consumed)

  • Amanita Muscaria (Amazing space)

  • Cannabis (My staple)

  • Ketamine (What a beautiful combination)

  • LSD (Smoking Harmala and/or 4-AcO-DMT on the peak of an acid trip is gorgeous. It sends the LSD into a frenzy. Not recommended for beginners.)

  • MDMA (As long as no MAOi was consumed)

  • Mescaline (As long as no MAOi was consumed)

  • Psilocybin

  • Salvia Divinorum I’ve combined a Salvia 20x Level 5 hit with 75mg 4-AcO-DMT fumarate in a bong, and then continued to smoke Salvia all through the night. Read about the most amazing and therapeutic trip on Salvia

  • Scopolamine (Not as much fun as I thought. Scopolamine is a dangerous substance, like Dutura, and it’s absolutely not recommended unless you know what you are doing. I’m very serious.)

Effects of 4-AcO-DMT (Physiological & Psychological)

The effects of 4-AcO-DMT are almost identical to Psilocybin. Since it is acetylated, it hits you faster and stronger than psilocybin. Mushrooms can take up to an hour and a half to take effect, whereas Psilacetin starts taking effect in 15-20 minutes after ingestion.

Many people have reported that 4-AcO-DMT begins in a way similar to how DMT begins. Many people report that it’s a much cleaner trip. The psychedelic community is seriously starting to consider it as a safe alternative to psilocybin mushrooms.

Physical Effects of 4-AcO-DMT

There are two primary effects I have noticed that are unique to 4-AcO-DMT.

  1. Extreme internal weather changes, ranging from a feeling of hypothermia to being really warm. Muscles spasm occur in my calf muscles and I am extremely uncomfortable for 15-20 minutes. This effect only happens when I vaporize 4-AcO-DMT. This effect does not happen when I eat or drink 4-AcO-DMT.

  2. Extreme Sedation and Yawning. No matter if I smoke, vaporize or eat 4-AcO-DMT, depending on dosage level I go through extreme yawning and feel very sedated and sleepy. The most I’ve ever taken at once time was 280mg of 4-AcO-DMT and I yawned hard for about an hour.

Other than these two effects, the rest is fairly identical to psilocybin.

Psychological Effects of 4-AcO-DMT

The psychological experience of 4-AcO-DMT is similar to Psilocybin Mushrooms apart from a few key things I noticed.

4-AcO-DMT is dark. Dark in the sense that it forces you to look at the darker parts of your psyche more so than mushrooms. After having consumed 10+ grams of 4-AcO-DMT over the last few years, compared to hundreds of mushroom journeys I’ve taken, I’ve noticed a difference in the mood.

4-AcO-DMT, to myself, seems to be more spiritual in nature. It has a unique … overtone… to the experience. Different strains of mushrooms seem to produce similar psychological experiences which are very slightly qualitatively different from each other.

For example, some people love Cubensis, and say that Golden Teacher or Golden Cap offers a slightly different experience than Mexicana etc. This is similar to how the same strain of Cannabis can affect two people completely differently.

4-AcO-DMT Tolerance

4-AcO-DMT produces a tolerance similar to LSD, which lasts up to two weeks, after which the tolerance resets back to baseline. It also similar to the tolerance produced by magic mushrooms and any other tryptamines.

Can You Microdose 4-AcO-DMT?

The happy answer is yes! And it works rather well. I’ve personally microdosed this substance for 2 years. In the spirit of keeping this article more well-managed, I’ve written a much more detailed guide on How To Microdose 4-AcO-DMT.

How does 4-AcO-DMT Compare to Psilocybin

Due to the acetylated functional group, it metabolizes faster than the dephosphorylation of Psilocybin. Where Psilocybin takes up to an hour and a half to metabolize the phosphoryloxy group (4-PO-DMT) into a Hydroxyl, the deacetylation of psilacetin is much faster.

Deacetylation of 4-AcO-DMT rapidly convert the Acetoxy group to a Hydroxyl (HO) in under 20 minutes. 4-AcO-DMT hits you harder and faster than mushrooms, however, the body load is slightly less that of Psilocin.

How Does 4-AcO-DMT Compare to DMT?

4-AcO-DMT is pure Acetylated Psilocin. It does not contain Baeocystine or Norbaeocystine as well as other alkaloids found in mushrooms. It begins similarly to how DMT begins.

You can rapidly tell the difference after the first minute in how clean this feels. It’s much less of a body load than DMT. On DMT the body is hammered hard instantly, but that’s not the case with 4-AcO-DMT.

Vaporizing 4-AcO-DMT

Vaporizing 4-AcO-DMT begins almost exactly like DMT and starts to become different after about 1-2 minutes.

A lot of people even say that 4-AcO-DMT is like a level 1 (weak) DMT trip that lasts several hours.


4-AcO-DMT is almost identical to Psilocybin. It’s called “mushroom powder by many, including myself. It’s consisent in dosage, and doesn’t vary in potency as pislocybin mushroom do. It’s safe to use as a mushroom alternative and I find it to be a lot more spiritual and clean than mushrooms.

It’s definitely an alternative for those who dislike mushrooms, but want the full mushroom experience. Remember to be safe out there and practice Harm Reduction though knowledge, information gathering, and by being aware of what you’re doing.

Even though the experience of 4-AcO-DMT can never be the same as “shrooms” due to the lack of many other alkaloids found in mushrooms, it’s almost identical in effect. Some might say it’s cleaner. 4-AcO-DMT hits you harder and faster than mushrooms do.


  1. https://erowid.org/chemicals/4_acetoxy_dmt/4_acetoxy_dmt_basics.shtml
  2. https://erowid.org/chemicals/4_acetoxy_dmt/4_acetoxy_dmt.shtml
  3. http://carbon.indstate.edu/rfitch/CHEM%20355/Acetylation%20Report%20Example.pdf
  4. https://psychonautwiki.org/wiki/4-AcO-DMT
  5. http://onlinelibrary.wiley.com/doi/10.1002/app.25743/abstract
  6. https://www.erowid.org/library/books_online/tihkal/tihkal18.shtml
  7. http://pac.iupac.org/publications/pac/pdf/2007/pdf/7911x1833.pdf
  8. https://en.wikipedia.org/wiki/Acetoxy_group

Hash C. Borgir

Hash is a software developer, tech geek, and psychedelic explorer with a passion for writing about plant medicines, psychology, consciousness, Linux, web development, hacking and science related topics.